Journal: Molecular Medicine Reports

Article Title: MicroRNA-217 is involved in the progression of atherosclerosis through regulating inflammatory responses by targeting sirtuin 1

doi: 10.3892/mmr.2019.10581

Figure Lengend Snippet: Effects of miR-217 inhibitor on SIRT1/AMPK-α/NF-κB pathway in THP-1 macrophages. THP-1 macrophages were pre-transfected with miR-217 inhibitor, inhibitor control, or miR-217 inhibitor + SIRT1-siRNA and treated with ox-LDL. (A) The protein levels of SIRT1, p-AMPK-α, AMPK-α, p-p65, and p65 in THP-1 macrophages was determined using western blotting. (B) the mRNA level of SIRT1 in THP-1 macrophages was determined using reverse transcription-quantitative PCR. The ratio of (C) p-AMPK-α/AMPK-α and (D) p-p65/p65 was calculated. **P<0.01 vs. Control; ## P<0.01 vs. ox-LDL; && P<0.01 vs. Inhibitor. AMPK-α, 5′-AMP-activated kinase α; miR-217, microRNA-217; ox-LDL, oxidized low density lipoprotein; p, phosphorylated; SIRT1, sirtuin 1.

Article Snippet: Following blocking in 5% skimmed milk at room temperature for 1.5 h, the membranes were incubated with primary antibodies against SIRT1 (120 kDa; 1:1,000; cat. no. 9475; Cell Signaling Technology, Inc.), phosphorylated (p)-AMP-activated protein kinase α (AMPK-α; 62 kDa; 1:1,000; cat. no. 50081; Cell Signaling Technology, Inc.), AMPK-α (62 kDa; 1:1,000; cat. no. 5831; Cell Signaling Technology, Inc.), p-NF-κB p65 (65 kDa; 1:1,000; cat. no. 3033; Cell Signaling Technology, Inc.), p65 (65 kDa; 1:1,000; cat. no. 8242; Cell Signaling Technology, Inc.) and β-actin (45 kDa; 1:1,000; cat. no. 4970; Cell Signaling Technology, Inc.) overnight at 4°C.

Techniques: Transfection, Control, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: The Journal of Physiology

Article Title: Post-exercise cold water immersion attenuates acute anabolic signalling and long-term adaptations in muscle to strength training

doi: 10.1113/JP270570

Figure Lengend Snippet: Change in phosphorylated p70S6KThr421/424 (A), phosphorylated p70S6KThr389 (B) and total p70S6K (C)

Article Snippet: The membrane was incubated overnight at 4°C with primary antibodies (from Cell Signaling Technology, Danvers, MA, USA, unless stated otherwise) against phosphorylated (p) p-p70S6 kinase Thr421/Ser424 (1:1000; no. 9204), p-p70S6 kinase Thr389 (1:1000; no. 9205), pERK-1 Thr202/Tyr204 and pERK2 Thr185/Tyr187 (1:1000; no. 4377), p-rpS6 Ser235/236 (1:2000; no. 2215S), p-rpS6 Ser240/244 (1:2000; no. 4856S) and total (T) proteins for T-p70S6 kinase (1:1000; no. 2708), T-ERK1/2 (1:1000; no. 4695), 4E-BP1 (1:1;000; no. 9644) and T-rpS6 (1:1000; Abcam, no. 40820).

Techniques: